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HISTOLOGICAL ASSESSMENT OF THE TESTES AND HORMONAL PROFILE OF ADULT MALE ALBINO WISTAR RATS FOLLOWING ORAL ADMINISTRATION OF MYRISTICA FRAGRANS (NUTMEG)

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HISTOLOGICAL ASSESSMENT OF THE TESTES AND HORMONAL PROFILE OF ADULT MALE ALBINO WISTAR RATS FOLLOWING ORAL ADMINISTRATION OF MYRISTICA FRAGRANS (NUTMEG) Empty HISTOLOGICAL ASSESSMENT OF THE TESTES AND HORMONAL PROFILE OF ADULT MALE ALBINO WISTAR RATS FOLLOWING ORAL ADMINISTRATION OF MYRISTICA FRAGRANS (NUTMEG)

Post by Admin 9th February 2020, 8:58 am

ABSTRACT
The study was carried out to assess the effect of oral
administration of Myristica fragrans (Nutmeg) on the histology of
the testis as well as its effects on hormonal profile (serum
testosterone). Thirty (30) adult male albino Wistar rats were used
for this study. The animals were randomly divided into six groups
of (A – F), with each group containing five rats respectively. Group
A (control group) received distilled water and 100 g of feed per
day for 26 days, group B received 1 g/kg body weight/day of
nutmeg mixed with 100 g of feed for 26 days, group C received 5
g/kg body weight of nutmeg per day mixed with 100 g of feed for
26 days, group D received 10 g/kg body weight of nutmeg per
day mixed with 100 g of feed for 26 days, group E received 15 g/
to 20 g/kg body weight of nutmeg per day mixed with 100 g of
feed for 26 days, group F received 20 g/kg body weight of
nutmeg per day mixed with 100 g of feed for 26 days. The
experiment lasted for 26 days. The rats were weighed before and
after the experiment. On the 26th day, the rats were sacrificed via
chloroform inhalation and the testes harvested and fixed
immediately in 10 % buffered formalin, processed and stained
with Harris hematoxylin and eosin (H&E) staining method. Blood
samples were collected by cardiac puncture in ETDA bottle and
plain bottles for analysis of serum testosterone. Data were
expressed as Mean + standard error of the Mean (Mean + SEM) and
subjected to one way analysis of variance (ANOVA). Significance
different between mean was assessed by student-New-Man-Keuls
post hoc test. 95 % level of significance (p < 0.05) was used for
statistical analysis and Microsoft Excel 2010 package was used for
graph and error bars. The result of this experiment revealed
persistent elevation in serum testosterone level in group (B – F)
test groups as compared to the control (Group A). Decrease
hepatic clearance of testosterone may be responsible for the
increase level of testosterone in the blood. Histological findings
from this study revealed that 10 g/kg body weight of nutmeg/day
– 20 g/kg body weight of nutmeg orally administered to male
albino Wistar rats have severe toxic effects on the histology of the
testis. This effect include cellular necrosis and degeneration of
spermatogenic, fibroblast and myoid cells. There is also severe
degeneration of the basement membrane of the testes. These
results revealed that consumption of higher dose of nutmeg have
cellular toxic effect on the testes and hence caution should be
applied on the intake of the spice nutmeg.
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Price: N2000.
Delivery time: 20 minutes after payment confirmation.
Type: Complete projects (chapters 1 - 5) plus preliminary pages.
Bonus: Even after purchase, we are open to help you through possible corrections by your supervisors.
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To purchase this project , transfer the sum of N2000 to the following details:
UBA Account Name: RichLord Jude
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Account Number: 2121101747.


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an email to juderichlord@gmail.com containing your payment confirmation details (and your department, including the account name you used to do the transaction), and the desired topic you have chosen.

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receive the project in few minutes sent to the same email you used to send the message.
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Join date : 2019-11-26
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